 |
|
|
|
The experimental animal facility plays an important role in the substitution of clinical trail. Animal room is classified as significance system that conducts on stabilizing experiment.
|
|
|
|
- Inside the building Type
- Container Type
|
|
|
|
In accordance with GLP(Good Laboratory Practice), it is necessary to follow the standards when conducting animal experiments.
The strict regulations are need for verifying the validity of experiments in the medical substance.
FDA have implemented a law to regulate stability assessment since 1979. In addition, legislation about GLP has been enacted by authorities of Japan in 1983.
GLP¡¯s standard includes managing animal experiments, regulating a structure of animal room.
|
|
|
Environmental condition criterion |
|
Animals Category
|
mouse
|
Rats
|
Hamster
|
Marmot
|
Rabbit
|
Monkey
|
Cat
|
Dog
|
Temperature
|
20 ~ 26¡É
|
18 ~ 28¡É
|
Humidity
|
(To be not less than 30%, 70% or more) from 40 to 60%
|
Cleanliness
|
class 10,000 (Barrier zone is not
breeding animals)
|
Cleanliness
|
Dust
|
class 10,000 (Barrier zone is not
breeding animals)
|
Drop bacteria
|
3 Below(Barrier zone is not breeding animals)
|
30 Below
|
30 Below (Animal breeding areas not normally)
|
Smell
|
Does not exceed the ammonia concentration 20ppm
|
Flow rate
|
13~18§¯/sec
|
Like
|
5mmH2O increases in static pressure difference. Higher 15mmH2O (SPF barrier zone) at constant
pressure difference (isolators)
|
|
150pa higher than the static pressure
difference around the hall (Isolrator)
|
Ventilation frequency
|
6 to 15 times / h (depending on the supply and exhaust change method)
|
Roughness
|
150 ~ 300 Lux (bottom 40 ~ 85 cm)
|
Noise
|
Does not exceed 60 dB (A) to
|
|
|
- Class nine minutes of the
classification of NASA
- Kord 9cm diameter opening 30 minutes (blood agar 48 h)
|
|
|
|
Category
|
Temperature(¡É)
|
Humidity(£¥)
|
Weight(g)
|
Occupied space(§©)
|
Ventilation(§©/h)
|
Mice
|
18~26
|
40~60
|
22
|
9.62*10-4
|
0.25
|
Hamster
|
18~26
|
40~70
|
120
|
1.87*10-3
|
0.7
|
Rabbit
|
18~21
|
40~60
|
~3600
|
0.116
|
21
|
Cat
|
18~29
|
30~70
|
~3700
|
0.17
|
21
|
mouse
|
18~26
|
40~70
|
250
|
3.34*10-3
|
1.4
|
Dog
|
18~29
|
30~70
|
~22,700
|
1.27
|
128
|
Primates
|
18~29
|
30~70
|
~5450
|
0.283
|
31
|
|
|
|
Temperature & Moisture conditions depending on species |
|
References animal kind
|
ASHRAE
|
Animal
Research Facility
|
G.L.P
|
Temperature
(¡É DB)
|
Humidity
(% RH)
|
Temperature
(¡É DB)
|
Humidity
(% RH)
|
Temperature
(¡É DB)
|
Humidity
(% RH)
|
Mouse
|
18 ~ 26
|
40 ~ 70
|
20 ~ 26
|
40 ~ 60
|
20 ~ 26
|
46 ~ 60
|
Rat
|
18 ~ 26
|
40 ~ 70
|
20 ~ 26
|
40 ~ 60
|
20 ~ 26
|
46 ~ 60
|
Hamster
|
18 ~ 26
|
40 ~ 70
|
20 ~ 26
|
40 ~ 60
|
20 ~ 26
|
46 ~ 60
|
Marmot
|
18 ~ 26
|
40 ~ 70
|
20 ~ 26
|
40 ~ 60
|
20 ~ 26
|
46 ~ 60
|
Rabbit
|
16 ~ 21
|
40 ~ 45
|
18 ~ 28
|
40 ~ 60
|
18 ~ 28
|
46 ~ 60
|
Cat
|
18 ~ 29
|
40 ~ 45
|
18 ~ 28
|
40 ~ 60
|
18 ~ 28
|
46 ~ 60
|
Dog
|
18 ~ 29
|
40 ~ 55
|
18 ~ 28
|
40 ~ 60
|
18 ~ 28
|
46 ~ 60
|
Primates
|
18 ~ 29
|
40 ~ 60
|
18 ~ 28
|
40 ~ 60
|
18 ~ 28
|
46 ~ 60
|
|
|
- ASHRAE : Handbook, 1982 Applications , 1982, p.14. 14
- Laboratory Animal Research Facility Based on
Laboratory Animal Facilities Construction and Equipment Guide
Line, 1983
|
|
|
Standards for Bio clean room. (NHB-5340-2) |
|
Geupbyeol
|
Particle
|
Biological particles
|
Diameter(um)
|
Cumulative particle
(Pc / §¤)
|
Part flow (improved / §¤)
|
Precipitation amount
(dog / §³)
|
NASA
|
209B
|
NASA
|
209B
|
NASA
|
209B
|
NASA
|
209B
|
100
|
¡Ã 0.5
|
¡Ã 0.5
|
¡Ã 3.5
|
¡Ã 3.5
|
0.0035
|
0.0035
|
12,900
|
12,900
|
10,000
|
¡Ã 0.5
|
¡Ã 0.5
|
¡Â 350
|
¡Â 350
|
0.0176
|
0.0176
|
64,600
|
64,600
|
¡Ã 5.0
|
¡Ã 5.0
|
¡Â 2.3
|
¡Â 2.3
|
100,000
|
¡Ã 0.5
|
¡Ã 0.5
|
¡Â 3,500
|
¡Â 3,500
|
0.0884
|
0.0884
|
323,000
|
323,000
|
¡Ã 5.0
|
¡Ã 5.0
|
¡Â 25
|
¡Â 25
|
|
Geupbyeol
|
Pressure (mmAq)
|
Temp( ¡É)
|
Humidity( £¥)
|
Airflow
(ventilation frequency)
|
Illuminance (Lux)
|
100
|
1.3 or later
|
Not political
|
40~45
|
Laminar way
|
1,080~1,620
|
10,000
|
0.45
±0.1m/s
|
|
100,000
|
Turbulence scheme ¡Ã 28 times / hour
|
|
|
|
|
|
|
- Breeding room : Lighting is controlled automatically depending on day and night
- Vestibule : establishment of buffer zone
- Corridor : to facilitate movement / more than 1.3meters are needed
- Clean preparation room : sterilization room. high pressure steam sterilizers, and Pass box are installed
- Air shower area : Installed in accordance with regulations such as air attachment, air current, and inter lock etc
- Sterilization room : Installed with abrasion&chemical resistance materials to reduce contamination
- Operating room : It is common to install the asepsis operating room
- Recovery room : accessibility options
|
|
|
Assembled & container type
|
|
|
|
- Control temperature and moisture accurately by Hot Gas By Pass
- 24hours, 24steps, and 24sets are settable
- Constant wind speed
- Separated type shelves
- Classified as significance system that conducts on stabilizing experiment.
|
|

|
|